Circulating Nucleic Acids in Plasma and Serum

Circulating Nucleic Acids in Plasma and Serum

This volume encompasses the proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held from the 9th to the 11th of November 2009 in Hong Kong.

Author: Peter B. Gahan

Publisher: Springer Science & Business Media

ISBN: 9048193826

Category: Medical

Page: 285

View: 783

DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.
Categories: Medical

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.

Author: Dave S. B. Hoon

Publisher:

ISBN: UVA:X004817516

Category: Medical

Page: 325

View: 186

Numerous questions remain regarding the origin, release, clearance, clinical utility, and possible functionality of circulating nucleic acids. Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.
Categories: Medical

Circulating Nucleic Acids in Plasma Or Serum

Circulating Nucleic Acids in Plasma Or Serum

This volume, taken from the proceedings of a conference in August 1999, discusses such issues.

Author: Philippe Anker

Publisher:

ISBN: 157331269X

Category: Science

Page: 188

View: 113

Free DNA present in both healthy and diseased human plasma has been found to express specific point mutations that may serve as diagnostic signposts - perhaps to reliable, non-invasive tests for breast and lung cancer, haematopoetic malignancies, colorectal or pancreatic carcinoma, and other tumours. In another research area, foetal DNA has been detected in maternal serum which presents an early-horizon alternative in utero test for gender. A parallel discovery of rearranged immunoglobin heavy-chain DNA in the plasma of patients wit Hodgkin's disease or acute B cell leukaemia has also emerged. These fast-emerging indicators suggest that it is time to standardize research techniques and develop protocols that can take a measure of circulating DNA's usefulness as a diagnostic tool. This volume, taken from the proceedings of a conference in August 1999, discusses such issues.
Categories: Science

Circulating Nucleic Acids in Serum and Plasma CNAPS IX

Circulating Nucleic Acids in Serum and Plasma     CNAPS IX

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids.

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9783319420448

Category: Medical

Page: 220

View: 258

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them.
Categories: Medical

Circulating Nucleic Acids in Plasma and Serum V

Circulating Nucleic Acids in Plasma and Serum V

This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter ...

Author: Peter B. Gahan

Publisher:

ISBN: STANFORD:36105132368627

Category: Blood

Page: 345

View: 836

Categories: Blood

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Author: Dave S. B. Hoon

Publisher:

ISBN: UVA:X004817516

Category: Medical

Page: 325

View: 356

Numerous questions remain regarding the origin, release, clearance, clinical utility, and possible functionality of circulating nucleic acids. Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.
Categories: Medical

Circulating Nucleic Acids in Early Diagnosis Prognosis and Treatment Monitoring

Circulating Nucleic Acids in Early Diagnosis  Prognosis and Treatment Monitoring

FEBS Lett 581:795–799 Tsang JC, Lo YM (2007) Circulating nucleic acids in
plasma/serum. Pathology 39:197–207 Wagner J (2012) Free DNA – new
potential analyte in clinical laboratory diagnostics? Biochem Med (Zagreb) 22:24
–38 El ...

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9789401791687

Category: Medical

Page: 471

View: 840

DNA and RNA fractions can be isolated from a variety of body fluids including whole blood, serum, plasma, urine, saliva and cerebrospinal fluid from both patients and healthy individuals. Such isolates can be exploited in the early detection of clinical disorders, stratification of patients for treatment, treatment monitoring and clinical follow-up. In addition, the use in fetal medicine allows the early detection of fetal sex, Rh factor and aneuploid disorders as well as following both fetal and premature born infant development. This volume is intended as a primer for those who are interested in entering the field of circulating nucleic acids. The areas covered in this volume include: · Background and general biology of circulating nucleic acids · Methodology · Applications of circulating nucleic acids · Quality Assurance · Ethics
Categories: Medical

Extracellular Nucleic Acids

Extracellular Nucleic Acids

Clin Cancer Res 11:1219–1225 Gahan PB (2008) Circulating nucleic acids in
plasma and serum: roles in diagnosis and prognosis in diabetes and cancer.
Infect Disord Drug Targets 8:100–108 Gahan PB, Chayen J (1965) Cytoplasmic ...

Author: Yo Kikuchi

Publisher: Springer Science & Business Media

ISBN: 3642126170

Category: Science

Page: 232

View: 586

Extracellular nucleic acids have recently emerged as important players in the fields of biology and the medical sciences. In the last several years, extracellular nucleic acids have been shown to be involved in not only microbial evolution as genetic elements but also to have structural roles in bacterial communities, such as biofilms. Circulating DNA and RNA have been found in human blood and expected to be useful as non-invasive markers for the diagnosis of several diseases. In addition, extracellular nucleic acids have attracted attention as active modulators of the immune system of higher organisms, including humans. This book covers nearly all of the newly developing fields related to extracellular nucleic acids, including those of basic biology, ecology and the medical sciences, and provides readers with the latest knowledge on them.
Categories: Science

Advances in Blood Research and Application 2013 Edition

Advances in Blood Research and Application  2013 Edition

However, a small amount of nucleic acids can also be found circulating freely in
the blood. The term 'Circulating Nucleic Acids' (CNA) refers to these segments of
DNA or RNA found in the bloodstream, plasma, serum or other body fluid. “There
 ...

Author:

Publisher: ScholarlyEditions

ISBN: 9781481694063

Category: Medical

Page: 656

View: 257

Advances in Blood Research and Application / 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Plasma. The editors have built Advances in Blood Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Plasma in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in Blood Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Categories: Medical

Handbook of Nucleic Acid Purification

Handbook of Nucleic Acid Purification

16.1.1 CELL-FREE DNA IN PLASMA AND SERUM The presence of circulating
nucleic acids in blood plasma was first described by Mandel and Métais in 1947 [
1], who demonstrated that extracellular nucleic acids could be detected in the ...

Author: Dongyou Liu

Publisher: CRC Press

ISBN: 9781420070972

Category: Science

Page: 584

View: 512

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods. It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter in this handbook: Explores the biological background important to understanding specific organisms and specimens Reviews principles and current techniques for efficient isolation Discusses challenges and future trends relating to improved recovery of nucleic acids Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.
Categories: Science

Development and Application of Biomarkers

Development and Application of Biomarkers

Goebel, G., Zitt, M., Zitt, M., and Müller, H.M., Circulating nucleic acids in plasma
or serum (CNAPS) as prognostic and predictive markers in patients with solid
neoplasias, Dis. Markers 21, 105–120, 2005. Sueoka, E., Sueoka, N., Iwanaga, K
. et ...

Author: Roger L. Lundblad

Publisher: CRC Press

ISBN: 1439819807

Category: Medical

Page: 297

View: 507

First introduced to biomedical research in 1980, the term biomarker has taken on a life of its own in recent years and has come to mean a number of things. In biomedical science, biomarker has evolved to most commonly mean a characteristic that can be used either as a diagnostic or a prognostic, but most significantly as a screening indicator for pathologies that tend to be somewhat silent prior to overt clinical display. Applying scientific rigor, as well as a disciplined approach to nomenclature, Roger Lundblad’s Development and Application of Biomarkers rationalizes the current enthusiasm for biomarkers with the use of well-established clinical laboratory analytes in clinical medicine. Highly respected for his work as both a classical protein scientist and as a pioneer in proteomics, Dr. Lundblad catalogs various biomarkers recognized in clinical medicine and, where possible, matches the expectations for advances in screening technologies with the realities of statistical analysis. More specifically, this important reference: Details an extensive list of biomarkers for various stages of a number of cancer types including ovarian, pancreatic, prostate, and breast cancer Looks at how proteomics is used for the discovery and validation of biomarkers Explores the use of microarray technology, ultra-high performance liquid chromatography, and computational bioinformatic approaches for the discovery and use of biomarkers Examines the use of cells and cell fragments as more complex biomarkers Organizes a host of significant biomarkers and essential research by type and use in a series of readily accessible tables Throughout this volume, Dr. Lundblad encourages consideration of biomarkers more as a concept than as laboratory analytes, emphasizing the relation between the discovery of a biomarker and the biology underlying its production. Ultimately, it is a thorough understanding of that underlying biology that will lead to the development of assays that are robust and reproducible, as well as clinically significant.
Categories: Medical

Tietz Textbook of Clinical Chemistry and Molecular Diagnostics E Book

Tietz Textbook of Clinical Chemistry and Molecular Diagnostics   E Book

We are devoting this section to discuss these issues by using as an example the
extraction of circulating nucleic acids in ... In this regard, plasma is a preferred
specimen type over serum because excessive background nucleic acids from ...

Author: Carl A. Burtis

Publisher: Elsevier Health Sciences

ISBN: 9781455759422

Category: Medical

Page: 2256

View: 726

As the definitive reference for clinical chemistry, Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th Edition offers the most current and authoritative guidance on selecting, performing, and evaluating results of new and established laboratory tests. Up-to-date encyclopedic coverage details everything you need to know, including: analytical criteria for the medical usefulness of laboratory procedures; new approaches for establishing reference ranges; variables that affect tests and results; the impact of modern analytical tools on lab management and costs; and applications of statistical methods. In addition to updated content throughout, this two-color edition also features a new chapter on hemostasis and the latest advances in molecular diagnostics. Section on Molecular Diagnostics and Genetics contains nine expanded chapters that focus on emerging issues and techniques, written by experts in field, including Y.M. Dennis Lo, Rossa W.K. Chiu, Carl Wittwer, Noriko Kusukawa, Cindy Vnencak-Jones, Thomas Williams, Victor Weedn, Malek Kamoun, Howard Baum, Angela Caliendo, Aaron Bossler, Gwendolyn McMillin, and Kojo S.J. Elenitoba-Johnson. Highly-respected author team includes three editors who are well known in the clinical chemistry world. Reference values in the appendix give you one location for comparing and evaluating test results. NEW! Two-color design throughout highlights important features, illustrations, and content for a quick reference. NEW! Chapter on hemostasis provides you with all the information you need to accurately conduct this type of clinical testing. NEW! Six associate editors, Ann Gronowski, W. Greg Miller, Michael Oellerich, Francois Rousseau, Mitchell Scott, and Karl Voelkerding, lend even more expertise and insight to the reference. NEW! Reorganized chapters ensure that only the most current information is included.
Categories: Medical

Intracranial Pressure and Brain Monitoring XII

Intracranial Pressure and Brain Monitoring XII

Circulating nucleic acid analysis: diagnostic applications for acute pathologies
1Department of Chemical Pathology, ... of molecular diagnostic strategies based
on the analysis of DNA/RNA molecules that are present in the plasma/serum of ...

Author: Wai S. Poon

Publisher: Springer Science & Business Media

ISBN: 9783211323182

Category: Medical

Page: 490

View: 898

88 short papers originating from the 12th International Symposium on Intracranial Pressure and Brain Monitoring held in August 2004 in Hong Kong present experimental as well as clinical research data on invasive and non-invasive intracranial pressure and brain biochemistry monitoring. The papers have undergone a peer-reviewing and are organized in nine sections: ICP management in head injury, neurochemical monitoring, intracranial hypertension, neuroimaging, hydrocephalus, clinical trails, experimental studies, brain compliance and biophysics.
Categories: Medical

Molecular Diagnosis of Cancer

Molecular Diagnosis of Cancer

Circulating nucleic acids refer to the DNA and RNA species that are present in
the plasma or serum portion of a blood sample. Although the existence of
circulating nucleic acids was described more than half a century ago, the
potential clinical ...

Author: J. E. Roulston

Publisher: Springer Science & Business Media

ISBN: 9781592597604

Category: Medical

Page: 393

View: 151

This volume represents a diverse collection of readily reproducible methods for use in cancer detection. Highlights include FISH-based methodologies currently used in the diagnosis of solid tumors, the molecular diagnosis of genetic abnormalities by DNA array technologies-including sequence-specific oligonucleotide arrays and CGH arrays-and methodologies directed at the detection of epigenetic events and at quantitative gene expression.
Categories: Medical

Methods of Cancer Diagnosis Therapy and Prognosis

Methods of Cancer Diagnosis  Therapy and Prognosis

The remaining contaminants and enzyme inhibitors are further removed by
centrifugation in two wash steps, and the circulating nucleic acids are eluted in
low-salt buffer. DNA from up to 200 μl plasma or serum samples can be isolated
using ...

Author: M. A. Hayat

Publisher: Springer Science & Business Media

ISBN: 9781402083693

Category: Medical

Page: 728

View: 327

The enormity of the global healthcare costs vical. One-fifth of all cancers worldwide as a result of cancer infliction cannot be are caused by a chronic infection, for overemphasized. There are more than 100 example, human papilloma virus (HPV) types of cancers; any part of the body can causes cervical cancer and hepatitis B be affected. More than 11 million people virus (HBV) causes liver cancer. Tobacco are diagnosed with cancer every year, and use is the most common preventable cause it is estimated that there will be 16 mil- of cancer in the world. Approximately, lion new cases per year by the year 2020. 168,000 cancer deaths are expected to be In 2005, 7. 6 million people died of can- caused by tobacco use. Approximately, cer, that is, 13% of the 58 million deaths 40% of cancer could be prevented, mainly worldwide. It is estimated that 9 million by not using tobacco, having a healthy people will die from cancer worldwide in diet, being physically active, preventing 2015 and 11. 4 million will die in 2030. infections that may cause cancer, reduc- More than 70% of all cancer deaths occur ing exposure to sunlight, and avoidance of in low and middle income countries. excessive alcohol consumption and stress Five major cancer causing overall mor- (anger). A third of cancers could be cured talities per year worldwide are (WHO): if detected early and treated adequately. It is well established that scientific 1. Lung: 1.
Categories: Medical

Cell and Tissue Based Molecular Pathology E Book

Cell and Tissue Based Molecular Pathology E Book

Taback B, Hoon DS: Circulating nucleic acids and proteomics of plasma/ serum:
Clinical utility. Ann NY Acad Sci 2004;1022:1–8. Traweek ST, Liu J, Battifora H:
Keratin gene expression in nonepithelial tissues: Detection with polymerase
chain ...

Author: Raymond R. Tubbs

Publisher: Elsevier Health Sciences

ISBN: 9781437719482

Category: Medical

Page: 452

View: 292

This volume in the Foundations in Diagnostic Pathology Series packs today's most essential cell and tissue base molecular pathology into a compact, high-yield format! It focuses on the state of the art in practical validated molecular diagnostics as applied across the fields of surgical pathology and cytology. With an emphasis on current, clinically valid, and diagnostically important applications today and in the near future, you can be assured you’re getting the most up-to-date, authoritative coverage available. Its pragmatic, well-organized approach, nearly 250 full-color illustrations, and at-a-glance boxes and tables make the information you need easy to access. Practical and affordable, this resource is ideal for study and review as well as everyday clinical practice! Offers detailed discussions on today’s technologies to help you select the best test for case evaluation. Presents recognized molecular pathologists who convey the most current information, keeping you on the cusp of your field. Features nearly 250 full-color illustrations that present important pathologic features, enabling you to form a differential diagnosis and compare your findings with actual cases. Uses a consistent, user-friendly format, including at-a-glance boxes and tables for easy reference.
Categories: Medical

The origin function and diagnostic potential of extracellular microRNA in human body fluids

The origin  function and diagnostic potential of extracellular microRNA in human body fluids

Thus, when investigating circulating miRNA in blood plasma and serum it is
critical to avoid the hemolysis and to account for all ... Enormous potential of
circulating nucleic acids for the prediction of a wide range of diseases is also
mentioned.

Author: Andrey Turchinovich

Publisher: Frontiers E-books

ISBN: 9782889192427

Category:

Page:

View: 248

Short non-coding RNA molecules, microRNAs (miRNAs), post-transcriptionally regulate gene expression in living cells. In recent years, miRNAs have been found in a wide spectrum of mammalian body fluids including blood plasma, saliva, urine, milk, seminal plasma, tears and amniotic fluid as extracellular circulating nuclease-resistant entities. The changes in miRNA spectra observed in certain fluids correlated with various pathological conditions suggesting that extracellular miRNAs can serve as informative biomarkers for certain diseases including cancer. However, the mechanism of generation and a biological role of extracellular miRNAs remain unclear. The current theories regarding extracellular miRNA origin and function suggest that these miRNAs can be either non-specific ‘by-products’ of cellular activity and cell death or specifically released cell-cell signaling messengers. The goal of this Research Topic is to bring together up-to-date knowledge about the extracellular miRNA and its role in disease diagnostics and, possibly, inter-cellular communication.
Categories:

Annual Review of Nursing Research Volume 25 2007

Annual Review of Nursing Research  Volume 25  2007

Circulating nucleic acids and proteomics of plasma/ serum: Clinical utility. Annals
of the New York Academy of Science, 1022, 1–8. Thompson, E. E., Kuttab-Boulos
, H., Witonsky, D., Yang, L., Roe, B. A., & Di Rienzo, A. (2004). CYP3a variation ...

Author:

Publisher: Springer Publishing Company

ISBN: 0826141390

Category: Medical

Page: 392

View: 450

This 25th anniversary edition of the Annual Review of Nursing Research is focused on nursing science in vulnerable populations. Identified as a priority in the nursing discipline, vulnerable populations are discussed in terms of the development of nursing science, diverse approaches in building the state of the science research, integrating biologic methods in the research, and research in reducing health disparities. Topics include: Measurement issues Prevention of infectious diseases among vulnerable populations Genomics and proteomics methodologies for research Promoting culturally appropriate interventions Community-academic research partnerships with vulnerable populations Vulnerable populations in Thailand: women living with HIV/AIDS As in all volumes of the Annual Reviews, leading nurse researchers provide students, other researchers, and clinicians with the foundations for evidence-based practice and further research.
Categories: Medical